2. Instruments, Products & Synthesis
  3. HPLC Columns
  4. Optical Isomer Separation Columns SUMICHIRAL
  5. Inversion of Elution Order

Inversion of Elution Order

In the SUMICHIRAL OA series, low molecular weight optically active compounds are immobilized onto the stationary phase.
By immobilizing an enantiomer of this optically active component, the elution order of the target substance can be inversed.
(Excluding OA-4800/4900/7000 series, 8000, AGP, CBH, and HSA)
The quantitation accuracy can be improved by eluting trace amount components first.

Example: N-Dansylvaline
Column SUMICHIRAL OA-3300、OA-3300S (4.6mm i.d.×250mm)
Mobile phase 0.01mol/L ammonium acetate in methanol
Flow rate 1.0mL/min
Detection wavenumber UV 254nm

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