| This equipment is designed based on the matrix
assisted laser desorption ionization (MALDI) and the time-of-flight mass spectrometry
(TOF/MS). The equipment is capable of ionizing polymer compounds (peptide, protein,
polysaccharides, synthesized polymers, etc.) and substances difficult to volatilize
without decomposing them and determining their molecular weight quickly with high
sensitivity. |
| When laser is irradiated pulsatingly
to a sample mixed with a matrix (sinapic acid, gentisic acid, etc.), the matrix
absorbs the laser energy, and the sample is rapidly heated and vaporized (ionized).
The generated ions are accelerated by a certain voltage and fly in a space without
electric fields. The ions reach the detector in the ascending order of molecular
weight. The molecular weight is determined by measuring the time of flight. |
| Fig.1 |
|
An example of measurement of immune
globulin IgG. The molecular ions are clearly observed in the sample having a molecular
weight of approx. 140 thousand. The equipment can easily measure samples having
a molecular weight of more than 100 thousand and is capable of measuring samples
having a molecular weight of up to 300 thousand. |
|
Fig.1 Innume globulin IgG
Matrix: Sinapic acid |
| Fig.2 |
|
An example of measurement of bovine
serum albumin. In addition to molecular ions, trimer cluster ions are detected
in the sample having a molecular weight of approx. 200 thousand. |
Fig.
2 Bovine serum albumin
Matrix: Sinapinic acid Negative ion measurement |
| Fig.3 |
|
An example of measurement of mixed sample of
peptide and protein. |
|
|
Fig.3 Mixed sample of peptide and protein
Matrix: Sinapic acid |
| Fig.4 |
|
An example of measurement of oligosaccharide
mixture. A peak consisting of several components differing in polymerization degree
is clearly observed. |
Fig.4 Oligosaccharide mixture
Matrix: Gentisic acid |
| Fig.5 |
|
An example of measurement of methotrexate,
one of antitumor drugs. Molecular ions are clearly observed in the sample having
a molecular weight of 454. The equipment is capable of measuring such low-molecular
weight compounds. |
Fig.5 Methotrexate
Matrix:2-(4-Hydroxyoheny lazo)benzoic acid
|
|
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